Composite

Part:BBa_K2688012:Design

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-09-23)


LEE5_GFP_native


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 566
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1283
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 52
    Illegal BsaI.rc site found at 1209


Design Notes

This plasmid is a simple cloning of our host lab' pKK-LEE5-GFP into pSB1C3 with mutation to ensure RFC10 compatibility.


Source

LEE5 is a topic of research at the host lab, which provided us with the source plasmid pKK-LEE5-gfp, that was then cloned to BioBrick RFC10 standard into pSB1C3.

References